Hotspot

ndhC-trnV-UAC

LSC · 51676-52618 · 943 bp

Back to Eremochloa

Pi 0.00495

MarkerSeek score 60.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 7
pi: 0.00495
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	1054-1069 bp	1.0	80.8
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAAGTCC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.0	57.9	1147-1162 bp	1.0	80.4
ndhC-trnV-UAC_p3	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	1055-1070 bp	1.0	78.9
ndhC-trnV-UAC_p4	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1053-1068 bp	1.0	78.3
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAAGTCCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	58.3	57.9	1146-1161 bp	1.0	78.0