Hotspot

trnG-UCC-trnT-GGU

LSC · 13774-15769 · 1996 bp

Back to Eremochloa

Pi 0.00635

MarkerSeek score 56.90

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1996
variable_sites: 19
pi: 0.00635
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	2084-2116 bp	1.0	43.1
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	2054-2086 bp	1.0	42.9
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	2050-2082 bp	1.0	42.2
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	2051-2083 bp	1.0	42.2
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	39.1	45.0	57.3	56.7	2086-2118 bp	1.0	42.2