Hotspot

trnS-GCU-psbD

LSC · 7876-8899 · 1024 bp

Back to Eremochloa

Pi 0.00197

MarkerSeek score 65.20

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1126-1173 bp	1.0	82.5
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1125-1172 bp	1.0	82.2
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1122-1169 bp	1.0	81.9
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1109-1156 bp	1.0	81.3
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ATAAGCCGGACCATCCTACA	50.0	50.0	57.6	57.9	1112-1159 bp	1.0	79.5