Hotspot

trnK-UUU-rps16

LSC · 3948-4494 · 547 bp

Back to Eremochloa

Pi 0.0171

MarkerSeek score 41.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 14
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TGAGGATCAGTCGTGGTCTT	TCGAGCCGTATGAGGAGAAA	50.0	50.0	58.1	58.2	967-996 bp	1.0	78.7
trnK-UUU-rps16_p2	TGAGGATCAGTCGTGGTCTT	TTTCTCGAGCCGTATGAGGA	50.0	50.0	58.1	58.2	971-1000 bp	1.0	78.7
trnK-UUU-rps16_p3	AACCTTTCCCTGCATTAGGC	TCGAGCCGTATGAGGAGAAA	50.0	50.0	58.1	58.2	1192-1220 bp	1.0	78.0
trnK-UUU-rps16_p4	AACCTTTCCCTGCATTAGGC	TTTCTCGAGCCGTATGAGGA	50.0	50.0	58.1	58.2	1196-1224 bp	1.0	77.9
trnK-UUU-rps16_p5	AGGATCAGTCGTGGTCTTCT	TCGAGCCGTATGAGGAGAAA	50.0	50.0	57.8	58.2	965-994 bp	1.0	77.5