Hotspot

ycf1

SSC · 113213-118819 · 5607 bp

Back to Eranthis

Pi 0.276

MarkerSeek score 74.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5607
variable_sites: 1318
pi: 0.276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCACCAAACCATAGCTGTT	GACATACTGAAACGACTCCCA	45.0	47.6	58.0	57.4	445 bp	0.5	47.2
ycf1_p2	TGCACCAAACCATAGCTGTT	TGACATACTGAAACGACTCCC	45.0	47.6	58.0	57.4	446 bp	0.5	47.2
ycf1_p3	TGATTATTGAACCAGCCCGG	GACATACTGAAACGACTCCCA	50.0	47.6	57.9	57.4	496 bp	0.5	46.9
ycf1_p4	TGATTATTGAACCAGCCCGG	TGACATACTGAAACGACTCCC	50.0	47.6	57.9	57.4	497 bp	0.5	46.9
ycf1_p5	ACACCATCCAGTGCAAAGAG	GACATACTGAAACGACTCCCA	50.0	47.6	58.1	57.4	885 bp	0.5	46.5