Hotspot

ndhH

SSC · 119644-120825 · 1182 bp

Back to Eranthis

Pi 0.463

MarkerSeek score 79.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 447
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCGGGAGCTCAAAATTCGTT	ACCCCTATACTGAGATCGGC	45.0	55.0	57.8	58.1	1774 bp	0.5	58.0
ndhH_p2	TTCGGGAGCTCAAAATTCGT	ACCCCTATACTGAGATCGGC	45.0	55.0	57.8	58.1	1775 bp	0.5	58.0
ndhH_p3	TCGGGAGCTCAAAATTCGTT	ATACGTTGTTGTACCCCTGC	45.0	50.0	57.8	57.9	1567 bp	0.5	57.7
ndhH_p4	TTCGGGAGCTCAAAATTCGT	ATACGTTGTTGTACCCCTGC	45.0	50.0	57.8	57.9	1568 bp	0.5	57.7
ndhH_p5	TCGGGAGCTCAAAATTCGTT	CAACGAATCGCACGTAGAGA	45.0	50.0	57.8	58.1	1948 bp	0.5	57.7