Hotspot

ndhG

SSC · 123818-124351 · 534 bp

Back to Eranthis

Pi 0.509

MarkerSeek score 80.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 534
variable_sites: 236
pi: 0.509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AATCAAATTGCTTTGGGGCG	TAAAGAAAAGAACGGGCCCC	45.0	50.0	57.9	58.1	1105 bp	0.5	58.5
ndhG_p2	AATCAAATTGCTTTGGGGCG	AAGAACGGGCCCCTATTAGT	45.0	50.0	57.9	58.1	1098 bp	0.5	58.3
ndhG_p3	CCAAACTATGGGGTGGTGAG	TAAAGAAAAGAACGGGCCCC	55.0	50.0	57.9	58.1	909 bp	0.5	58.3
ndhG_p4	AATCAAATTGCTTTGGGGCG	GATCCGCTCATGAAAGAGGG	45.0	55.0	57.9	58.1	1742 bp	0.5	58.2
ndhG_p5	AATCAAATTGCTTTGGGGCG	GGATCCGCTCATGAAAGAGG	45.0	55.0	57.9	58.1	1743 bp	0.5	58.2