Hotspot

ndhE

SSC · 124609-124914 · 306 bp

Back to Eranthis

Pi 0.41

MarkerSeek score 77.20

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 118
pi: 0.41
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TGGACTGTTGGAGATGGAGT	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.0	58.0	1307 bp	0.5	59.7
ndhE_p2	TTCCGTCTTTGGACTGTTGG	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.0	58.0	1316 bp	0.5	59.7
ndhE_p3	TGGACTGTTGGAGATGGAGT	TCTTGGGGCAGAAGCAATTT	50.0	45.0	58.0	58.0	1210 bp	0.5	59.6
ndhE_p4	TGGACTGTTGGAGATGGAGT	TTCTTGGGGCAGAAGCAATT	50.0	45.0	58.0	58.0	1211 bp	0.5	59.6
ndhE_p5	TGGACTGTTGGAGATGGAGT	CTTAGCTTTACACCCGTCCC	50.0	55.0	58.0	58.0	1189 bp	0.5	59.6