Hotspot

ndhD

SSC · 125544-127046 · 1503 bp

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Pi 0.474

MarkerSeek score 79.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 576
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCATGAGACAACTCGTAGCA	CTGCAATTGTGGCTTCCATG	50.0	50.0	58.0	58.0	2032 bp	0.5	59.9
ndhD_p2	GCATGAGACAACTCGTAGCA	TCCTTTGGCGAAATCCAACA	50.0	45.0	58.0	58.0	2336 bp	0.5	59.9
ndhD_p3	AAATTGCTTCTGCCCCAAGA	CTGCAATTGTGGCTTCCATG	45.0	50.0	58.0	58.0	2129 bp	0.5	59.9
ndhD_p4	AATTGCTTCTGCCCCAAGAA	CTGCAATTGTGGCTTCCATG	45.0	50.0	58.0	58.0	2128 bp	0.5	59.9
ndhD_p5	GGGACGGGTGTAAAGCTAAG	CTGCAATTGTGGCTTCCATG	55.0	50.0	58.0	58.0	2150 bp	0.5	59.8