Hotspot

trnL-UAG-ndhF

SSC · 128499-129658 · 1160 bp

Back to Eranthis

Pi 0.445

MarkerSeek score 80.20

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: SSC
length_bp: 1160
variable_sites: 429
pi: 0.445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	ACAGCGTGTCTACCGATTTC	AGGAACTGGAAGGGGAATGA	50.0	50.0	58.0	57.9	1248 bp	0.5	59.5
trnL-UAG-ndhF_p2	AGCGTGTCTACCGATTTCAC	AGGAACTGGAAGGGGAATGA	50.0	50.0	58.0	57.9	1246 bp	0.5	59.5
trnL-UAG-ndhF_p3	GAACAGCGTGTCTACCGATT	AGGAACTGGAAGGGGAATGA	50.0	50.0	58.0	57.9	1250 bp	0.5	59.5
trnL-UAG-ndhF_p4	CAGCGTGTCTACCGATTTCA	AGGAACTGGAAGGGGAATGA	50.0	50.0	58.0	57.9	1247 bp	0.5	59.4
trnL-UAG-ndhF_p5	TCCACACAAAAGAGCTGCAT	AGGAACTGGAAGGGGAATGA	45.0	50.0	58.0	57.9	1903 bp	0.5	59.4