Hotspot

ndhE-psaC

SSC · 124915-125171 · 257 bp

Back to Eranthis

Pi 0.475

MarkerSeek score 76.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 114
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TTGCTATGGCTCGTCAATCC	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.0	58.0	1072 bp	0.5	59.6
ndhE-psaC_p2	TTGCTATGGCTCGTCAATCC	TCTTGGGGCAGAAGCAATTT	50.0	45.0	58.0	58.0	975 bp	0.5	59.5
ndhE-psaC_p3	TTGCTATGGCTCGTCAATCC	TTCTTGGGGCAGAAGCAATT	50.0	45.0	58.0	58.0	976 bp	0.5	59.5
ndhE-psaC_p4	TTGCTATGGCTCGTCAATCC	CTTAGCTTTACACCCGTCCC	50.0	55.0	58.0	58.0	954 bp	0.5	59.5
ndhE-psaC_p5	GGGGCCCGTTCTTTTCTTTA	TGCTACGAGTTGTCTCATGC	50.0	50.0	58.1	58.0	1006 bp	0.5	59.3