Hotspot

ndhG-ndhE

SSC · 124352-124608 · 257 bp

Back to Eranthis

Pi 0.416

MarkerSeek score 77.20

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 101
pi: 0.416
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	TGGACTGTTGGAGATGGAGT	TCTTGGGGCAGAAGCAATTT	50.0	45.0	58.0	58.0	1210 bp	0.5	59.6
ndhG-ndhE_p2	TGGACTGTTGGAGATGGAGT	TTCTTGGGGCAGAAGCAATT	50.0	45.0	58.0	58.0	1211 bp	0.5	59.6
ndhG-ndhE_p3	TGGACTGTTGGAGATGGAGT	CTTAGCTTTACACCCGTCCC	50.0	55.0	58.0	58.0	1189 bp	0.5	59.6
ndhG-ndhE_p4	TTCCGTCTTTGGACTGTTGG	TCTTGGGGCAGAAGCAATTT	50.0	45.0	58.0	58.0	1219 bp	0.5	59.6
ndhG-ndhE_p5	TTCCGTCTTTGGACTGTTGG	TTCTTGGGGCAGAAGCAATT	50.0	45.0	58.0	58.0	1220 bp	0.5	59.6