Hotspot

rps15-ndhH

SSC · 119467-119643 · 177 bp

Back to Eranthis

Pi 0.398

MarkerSeek score 77.00

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 177
variable_sites: 66
pi: 0.398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	TCGGGAGCTCAAAATTCGTT	CCTCTCCACCAATAATGCCC	45.0	55.0	57.8	58.0	836 bp	0.5	58.5
rps15-ndhH_p2	TTCGGGAGCTCAAAATTCGT	CCTCTCCACCAATAATGCCC	45.0	55.0	57.8	58.0	837 bp	0.5	58.5
rps15-ndhH_p3	TCGGGAGCTCAAAATTCGTT	CCCCTTCAACTCGTTCCAAA	45.0	50.0	57.8	58.0	815 bp	0.5	58.4
rps15-ndhH_p4	TTCGGGAGCTCAAAATTCGT	CCCCTTCAACTCGTTCCAAA	45.0	50.0	57.8	58.0	816 bp	0.5	58.4
rps15-ndhH_p5	TCGGGAGCTCAAAATTCGTT	ATCCAGCCATAGGGGAGATC	45.0	55.0	57.8	58.0	714 bp	0.5	58.3