Hotspot

rpl14-rpl16

LSC · 84491-84632 · 142 bp

Back to Eranthis

Pi 0.0945

MarkerSeek score 77.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 12
pi: 0.0945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CGCTGTTGTCCGCTACATTT	GCGTCCAAAATGCCTATACG	50.0	50.0	59.2	57.3	218-230 bp	1.0	73.3
rpl14-rpl16_p2	CGCTGTTGTCCGCTACATT	GCGTCCAAAATGCCTATACG	52.6	50.0	58.5	57.3	218-230 bp	1.0	70.6
rpl14-rpl16_p3	CGCTGTTGTCCGCTACATTT	ATAGCTGCGTCCAAAATGCC	50.0	50.0	59.2	59.3	224-236 bp	1.0	68.5
rpl14-rpl16_p4	CGCTGTTGTCCGCTACATTT	GCTGCGTCCAAAATGCCTAT	50.0	50.0	59.2	59.3	221-233 bp	1.0	68.5
rpl14-rpl16_p5	CGCTGTTGTCCGCTACATTT	CTATAGCTGCGTCCAAAATGC	50.0	47.6	59.2	57.7	226-238 bp	1.0	68.4