Hotspot

rpl16

LSC · 77229-78702 · 1474 bp

Back to Eragrostis

Pi 0.0194

MarkerSeek score 63.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1474
variable_sites: 111
pi: 0.0194
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	CCCTGTCTTCTCATTCTGGC	50.0	55.0	58.1	58.0	2030-2107 bp	0.631579	72.9
rpl16_p2	TCAGCAATAGTGTCCTTGCC	CCCTGTCTTCTCATTCTGGC	50.0	55.0	57.9	58.0	2240-2317 bp	0.631579	72.5
rpl16_p3	TCTCAATTCCTCGGCAATCG	CCTGTCTTCTCATTCTGGCC	50.0	55.0	58.1	58.0	2029-2106 bp	0.578947	70.3
rpl16_p4	TCAGCAATAGTGTCCTTGCC	CCTGTCTTCTCATTCTGGCC	50.0	55.0	57.9	58.0	2239-2316 bp	0.578947	69.9
rpl16_p5	TTTCTAGAGGCATTTGGGGC	CCTGTCTTCTCATTCTGGCC	50.0	55.0	57.9	58.0	1868-1945 bp	0.578947	69.9