Hotspot

psaC-ndhE

SSC · 108044-108558 · 515 bp

Back to Eragrostis

Pi 0.0225

MarkerSeek score 67.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 515
variable_sites: 46
pi: 0.0225
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGGTTGTTTCAGGCCCTAAA	ATCACAAGCCGAAACATGGT	50.0	45.0	58.0	58.1	892-1012 bp	1.0	87.8
psaC-ndhE_p2	ACACAATCTTCGGTTCTCGG	ATCACAAGCCGAAACATGGT	50.0	45.0	57.9	58.1	815-935 bp	0.947368	85.3
psaC-ndhE_p3	CGATTCCCAATCTCCAGTGG	ATCACAAGCCGAAACATGGT	55.0	45.0	58.0	58.1	1281-1401 bp	0.947368	84.9
psaC-ndhE_p4	ACAATCTTCGGTTCTCGGTG	ATCACAAGCCGAAACATGGT	50.0	45.0	57.9	58.1	894-909 bp	0.315789	55.2
psaC-ndhE_p5	CACAATCTTCGGTTCTCGGT	ATCACAAGCCGAAACATGGT	50.0	45.0	57.9	58.1	895-910 bp	0.315789	55.2