Hotspot

ccsA-ndhD

SSC · 105983-106175 · 193 bp

Back to Eragrostis

Pi 0.0494

MarkerSeek score 69.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 193
variable_sites: 30
pi: 0.0494
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAACTCTTTTGTGCGGATC	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	849-865 bp	1.0	88.3
ccsA-ndhD_p2	ACCTGCTCTCCAATCTCAGT	GGATTCTGGACCACGAGAAC	50.0	55.0	58.1	58.0	907-923 bp	1.0	87.6
ccsA-ndhD_p3	GCAACTCTTTTGTGCGGATC	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.0	57.9	1047-1063 bp	1.0	86.5
ccsA-ndhD_p4	ACCTGCTCTCCAATCTCAGT	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.1	57.9	1105-1121 bp	1.0	86.0
ccsA-ndhD_p5	GAAGCGTGGGGATCCTATTG	GGATTCTGGACCACGAGAAC	55.0	55.0	58.1	58.0	561-577 bp	0.947368	83.4