Hotspot

rpl32-trnL-UAG

SSC · 104518-104856 · 339 bp

Back to Eragrostis

Pi 0.0492
MarkerSeek score 73.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
339
variable_sites
64
pi
0.0492
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAACGAGCATCCAAAACC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.1 57.9 439-470 bp 0.947368 79.4
rpl32-trnL-UAG_p2 GGTAACGAGCATCCAAAACC ACTGAGATTGGAGAGCAGGT 50.0 50.0 57.1 58.1 960-991 bp 0.947368 78.6
rpl32-trnL-UAG_p3 GGTAACGAGCATCCAAAACC GATCCGCACAAAAGAGTTGC 50.0 50.0 57.1 58.0 1018-1049 bp 0.947368 78.5
rpl32-trnL-UAG_p4 CCAAAAGGTTTTTCTGGGCA GATCCGCACAAAAGAGTTGC 45.0 50.0 57.0 58.0 1000-1031 bp 0.947368 77.6
rpl32-trnL-UAG_p5 ACCAAAAGGTTTTTCTGGGC GATCCGCACAAAAGAGTTGC 45.0 50.0 57.0 58.0 1001-1032 bp 0.947368 77.6