Hotspot

ndhF-rpl32

SSC · 103340-104325 · 986 bp

Back to Eragrostis

Pi 0.032
MarkerSeek score 71.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
986
variable_sites
116
pi
0.032
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.93
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGATTACCCAGGCATATTGA TGACATCGAAGTACGTTTTT 45.0 35.0 54.5 53.2 961-1053 bp 1.0 52.0
ndhF-rpl32_p2 GGATTACCCAGGCATATTGA TTGACATCGAAGTACGTTTT 45.0 35.0 54.5 53.2 962-1054 bp 1.0 52.0
ndhF-rpl32_p3 GGATTACCCAGGCATATTGA TTTGACATCGAAGTACGTTT 45.0 35.0 54.5 53.2 963-1055 bp 1.0 52.0
ndhF-rpl32_p4 GGATTACCCAGGCATATTGA TTTTGACATCGAAGTACGTT 45.0 35.0 54.5 53.2 964-1056 bp 1.0 52.0
ndhF-rpl32_p5 GGATTACCCAGGCATATTGA TTTTTGACATCGAAGTACGT 45.0 35.0 54.5 53.2 965-1057 bp 1.0 52.0