Hotspot

petA-psbJ

LSC · 60577-61356 · 780 bp

Back to Eragrostis

Pi 0.027

MarkerSeek score 76.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 75
pi: 0.027
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	867-980 bp	0.947368	71.8
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	868-981 bp	0.947368	71.8
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	ACCGGTATTCTTGTGATTGGT	50.0	42.9	58.7	57.3	851-964 bp	1.0	71.6
petA-psbJ_p4	AGGCCTTTTGTTCTTTTTCGC	GCTGATAGGTACTGTAACCGG	42.9	52.4	58.2	57.6	861-974 bp	0.947368	67.9
petA-psbJ_p5	AGGCCTTTTGTTCTTTTTCGC	GGCTGATAGGTACTGTAACCG	42.9	52.4	58.2	57.6	862-975 bp	0.947368	67.9