Hotspot

trnC-GCA-rpoB

LSC · 18539-19547 · 1009 bp

Back to Eragrostis

Pi 0.0225

MarkerSeek score 72.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 98
pi: 0.0225
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	GTGGACATTCCCTCATTCCC	50.0	55.0	58.1	57.9	927-1177 bp	1.0	90.2
trnC-GCA-rpoB_p2	ATTTGATTGATCAGGCGGCA	GTGGACATTCCCTCATTCCC	45.0	55.0	58.2	57.9	967-1217 bp	1.0	88.7
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TGGACATTCCCTCATTCCCA	50.0	50.0	58.1	58.3	926-1176 bp	1.0	88.1
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	GGGGTATTGTGGACATTCCC	50.0	55.0	58.1	57.9	935-1185 bp	0.947368	87.8
trnC-GCA-rpoB_p5	ATTTGATTGATCAGGCGGCA	GGGGTATTGTGGACATTCCC	45.0	55.0	58.2	57.9	975-1225 bp	0.947368	86.3