Hotspot

petN-trnC-GCA

LSC · 17588-18467 · 880 bp

Back to Eragrostis

Pi 0.0266

MarkerSeek score 72.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 89
pi: 0.0266
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	520-929 bp	1.0	82.4
petN-trnC-GCA_p2	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	579-988 bp	0.947368	80.1
petN-trnC-GCA_p3	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	578-987 bp	0.947368	80.1
petN-trnC-GCA_p4	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	581-990 bp	0.947368	79.7
petN-trnC-GCA_p5	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	580-989 bp	0.947368	79.7