Hotspot

psbM-petN

LSC · 16682-17497 · 816 bp

Back to Eragrostis

Pi 0.0226

MarkerSeek score 69.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 75
pi: 0.0226
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACTGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	814-899 bp	1.0	79.9
psbM-petN_p2	AAAACTGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	813-898 bp	1.0	79.9
psbM-petN_p3	AAAACTGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	812-897 bp	1.0	79.4
psbM-petN_p4	AAAACTGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	872-957 bp	1.0	79.2
psbM-petN_p5	AAAACTGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	809-894 bp	1.0	79.1