Hotspot

trnT-GGU-trnE-UUC

LSC · 14435-14949 · 515 bp

Back to Eragrostis

Pi 0.0314
MarkerSeek score 69.00
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
515
variable_sites
60
pi
0.0314
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 ATCGCGGGCCTTTACTTTAG GCATCGACCCAGGAAGAATT 50.0 50.0 58.1 58.0 1343-1413 bp 1.0 91.2
trnT-GGU-trnE-UUC_p2 ATCGCGGGCCTTTACTTTAG TTGAACTACAATCCCTGCGG 50.0 50.0 58.1 57.9 1781-1848 bp 1.0 90.7
trnT-GGU-trnE-UUC_p3 GAACATTCTGAGCACGGGAA GCATCGACCCAGGAAGAATT 50.0 50.0 58.2 58.0 888-954 bp 0.578947 70.4
trnT-GGU-trnE-UUC_p4 ATCGCGGGCCTTTACTTTAG TGATATTGCCTTCTGCCCAC 50.0 50.0 58.1 57.9 1960-1974 bp 0.263158 52.4
trnT-GGU-trnE-UUC_p5 GAACATTCTGAGCACGGGAA TGATATTGCCTTCTGCCCAC 50.0 50.0 58.2 57.9 1506-1520 bp 0.263158 51.8