Hotspot

trnG-GCC-trnfM-CAU

LSC · 12343-12685 · 343 bp

Back to Eragrostis

Pi 0.0315
MarkerSeek score 72.10
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
343
variable_sites
52
pi
0.0315
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GCGAGCGTAGTTCAATGGTA AGTTTTTGGCCCTACAGAGC 50.0 50.0 58.1 58.1 493-518 bp 1.0 90.6
trnG-GCC-trnfM-CAU_p2 TAGCGAGCGTAGTTCAATGG AGTTTTTGGCCCTACAGAGC 50.0 50.0 57.8 58.1 495-520 bp 1.0 89.7
trnG-GCC-trnfM-CAU_p3 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 449-474 bp 1.0 89.5
trnG-GCC-trnfM-CAU_p4 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.1 58.3 464-489 bp 1.0 89.1
trnG-GCC-trnfM-CAU_p5 GCGAGCGTAGTTCAATGGTA CCAGTTTTTGGCCCTACAGA 50.0 50.0 58.1 57.7 495-520 bp 1.0 89.0