Hotspot

psbH-petB

Genome · 82355-83274 · 920 bp

Back to Equisetum

Pi 0.0488

MarkerSeek score 78.10

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: Genome
length_bp: 920
variable_sites: 116
pi: 0.0488
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	ATAGCGATCAAAGTTGCGGT	AAAGCTTCAGTAACCGTGGG	45.0	50.0	58.0	58.1	1279-1306 bp	0.909091	84.5
psbH-petB_p2	CGATCAAAGTTGCGGTTTCC	AAAGCTTCAGTAACCGTGGG	50.0	50.0	58.0	58.1	1275-1302 bp	0.909091	84.5
psbH-petB_p3	ATAGCGATCAAAGTTGCGGT	ACCAATCTGATCCCACGGTA	45.0	50.0	58.0	58.1	1514-1542 bp	0.909091	84.2
psbH-petB_p4	CGATCAAAGTTGCGGTTTCC	ACCAATCTGATCCCACGGTA	50.0	50.0	58.0	58.1	1510-1538 bp	0.909091	84.1
psbH-petB_p5	ATAGCGATCAAAGTTGCGGT	TCAGTAACCGTGGGACGATA	45.0	50.0	58.0	57.9	1273-1295 bp	0.545455	69.5