Hotspot

rps12-clpP

Genome · 77189-78179 · 991 bp

Back to Equisetum

Pi 0.0734

MarkerSeek score 79.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 991
variable_sites: 155
pi: 0.0734
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	AAAGCCCTCAGGACTTGTTG	CCGGAGCCGTATGATTTGAA	50.0	50.0	58.0	58.1	1708-1992 bp	0.909091	87.8
rps12-clpP_p2	AAAGCCCTCAGGACTTGTTG	AAGCCAGAGACTACGGACTT	50.0	50.0	58.0	58.1	1162-1445 bp	0.909091	87.7
rps12-clpP_p3	AAAGCCCTCAGGACTTGTTG	GTATGGGGTTAGCTGCATCC	50.0	55.0	58.0	58.1	1426-1709 bp	0.909091	87.4
rps12-clpP_p4	AAAGCCCTCAGGACTTGTTG	ATGTCAGCAGAAGAAGCCAG	50.0	50.0	58.0	57.9	1175-1458 bp	0.909091	87.4
rps12-clpP_p5	AAAGCCCTCAGGACTTGTTG	TTCATCAACCCGCTAGTTCG	50.0	50.0	58.0	58.0	1580-1618 bp	0.272727	62.4