Hotspot

psbD-trnT-GGU

Genome · 48350-49253 · 904 bp

Back to Equisetum

Pi 0.0952

MarkerSeek score 77.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 904
variable_sites: 206
pi: 0.0952
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TGTAGCACCGTGAATAGCAC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1853-1927 bp	0.818182	81.8
psbD-trnT-GGU_p2	TGTAGCACCGTGAATAGCAC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1817-1891 bp	0.818182	81.7
psbD-trnT-GGU_p3	ACCGATTAGACCGAAAGCAC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1574-1648 bp	0.636364	74.3
psbD-trnT-GGU_p4	GATTAGACCGAAAGCACCGT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.0	58.0	1571-1645 bp	0.636364	74.3
psbD-trnT-GGU_p5	GATTAGACCGAAAGCACCGT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1535-1609 bp	0.636364	74.2