Hotspot

trnY-GUA-trnE-UUC

Genome · 40246-44818 · 4573 bp

Back to Equisetum

Pi 0.189

MarkerSeek score 70.00

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: Genome
length_bp: 4573
variable_sites: 1878
pi: 0.189
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	GTCCAGAAATCGTACCACCA	GGTTGTGGGTACGACTTCTG	50.0	55.0	57.2	58.2	747-909 bp	0.272727	53.2
trnY-GUA-trnE-UUC_p2	GTCCAGAAATCGTACCACCA	GTTGTGGGTACGACTTCTGG	50.0	55.0	57.2	58.2	746-908 bp	0.272727	53.2
trnY-GUA-trnE-UUC_p3	GTCCAGAAATCGTACCACCA	CCATAGGTTGCAGGTACGAC	50.0	55.0	57.2	58.1	586-743 bp	0.181818	47.5
trnY-GUA-trnE-UUC_p4	TGTCCAGAAATCGTACCACC	CCATAGGTTGCAGGTACGAC	50.0	55.0	57.2	58.1	587-744 bp	0.181818	47.5
trnY-GUA-trnE-UUC_p5	GTCCAGAAACTGTACGGTCA	CCATAGGTTGCAGGTACGAC	50.0	55.0	57.2	58.1	710-862 bp	0.181818	46.9