Hotspot

trnK-UUU-chlB

Genome · 15879-16848 · 970 bp

Back to Equisetum

Pi 0.104

MarkerSeek score 77.30

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 970
variable_sites: 225
pi: 0.104
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	GATTCAAGCCTCACAAGCCT	CGTCATATTGGTAAGCGCCT	50.0	50.0	58.2	58.1	1661-1727 bp	1.0	89.6
trnK-UUU-chlB_p2	AACCCTTCTGAATTGGGCTG	CGTCATATTGGTAAGCGCCT	50.0	50.0	58.1	58.1	1453-1461 bp	0.363636	60.5
trnK-UUU-chlB_p3	AACCCTTCTGAATTGGGCTG	TCATATTGGTAAGCGCCTCG	50.0	50.0	58.1	57.9	1451-1459 bp	0.363636	60.4
trnK-UUU-chlB_p4	CTCGTTCCTCCGGTTTCTTT	CGTCATATTGGTAAGCGCCT	50.0	50.0	57.8	58.1	1721-1732 bp	0.363636	60.3
trnK-UUU-chlB_p5	CTCGTTCCTCCGGTTTCTTT	TCATATTGGTAAGCGCCTCG	50.0	50.0	57.8	57.9	1719-1730 bp	0.363636	60.2