primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	GCCAAAGCTTGTTGTCAACC	TCCAGGGTTTTTCATTTTGTTGT	20	23	50.0	34.8	58.4	57.6	13.1	0.0	3.857371	13426	15878	2887-2952	0.363636	31.2
trnK-UUU_p2	trnK-UUU	2	GCCAAAGCTTGTTGTCAACC	CCAGGGTTTTTCATTTTGTTGT	20	22	50.0	36.4	58.4	56.2	13.1	0.0	4.275050	13426	15878	2886-2951	0.363636	27.9
trnK-UUU_p3	trnK-UUU	3	GCCAAAGCTTGTTGTCAACC	ATCCAGGGTTTTTCATTTTGTTGT	20	24	50.0	33.3	58.4	58.0	13.1	0.0	4.429433	13426	15878	2888-2953	0.363636	26.6
trnK-UUU_p4	trnK-UUU	4	GCTGGTAATCCAATCGCAGA	TGGGGAAACTCTTTTGTAAGA	20	21	50.0	38.1	58.0	54.7	0.0	0.0	4.383924	13426	15878	2851-2900	0.181818	15.5
trnK-UUU_p5	trnK-UUU	5	GACGGTTTTCAGTGCTGGTA	TGGGGAAACTCTTTTGTAAGA	20	21	50.0	38.1	58.1	54.7	0.0	0.0	4.463785	13426	15878	2864-2913	0.181818	14.9
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	GATTCAAGCCTCACAAGCCT	CGTCATATTGGTAAGCGCCT	20	20	50.0	50.0	58.2	58.1	0.0	2.3	0.302555	15879	16848	1661-1727	1.000000	89.6
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	AACCCTTCTGAATTGGGCTG	CGTCATATTGGTAAGCGCCT	20	20	50.0	50.0	58.1	58.1	13.2	2.3	0.205267	15879	16848	1453-1461	0.363636	60.5
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	AACCCTTCTGAATTGGGCTG	TCATATTGGTAAGCGCCTCG	20	20	50.0	50.0	58.1	57.9	13.2	2.3	0.220047	15879	16848	1451-1459	0.363636	60.4
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	CTCGTTCCTCCGGTTTCTTT	CGTCATATTGGTAAGCGCCT	20	20	50.0	50.0	57.8	58.1	0.0	2.3	0.299454	15879	16848	1721-1732	0.363636	60.3
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	CTCGTTCCTCCGGTTTCTTT	TCATATTGGTAAGCGCCTCG	20	20	50.0	50.0	57.8	57.9	0.0	2.3	0.314234	15879	16848	1719-1730	0.363636	60.2
trnG-UCC_p1	trnG-UCC	1	TGGGTTCGGAATCGGAAATC	TTTCCTCCTCCATTCTCGGT	20	20	50.0	50.0	58.0	58.0	4.8	0.0	0.080059	21105	21817	1150-1165	0.363636	59.0
trnG-UCC_p2	trnG-UCC	2	TGGGTTCGGAATCGGAAATC	CGCTTTCCTCCTCCATTCTC	20	20	50.0	55.0	58.0	58.1	4.8	0.0	0.085936	21105	21817	1153-1168	0.363636	58.9
trnG-UCC_p3	trnG-UCC	3	TGGGTTCGGAATCGGAAATC	GCTTTCCTCCTCCATTCTCG	20	20	50.0	55.0	58.0	58.1	4.8	0.0	0.085936	21105	21817	1152-1167	0.363636	58.9
trnG-UCC_p4	trnG-UCC	4	TGGGTTCGGAATCGGAAATC	TCCTCCTCCATTCTCGGTAC	20	20	50.0	55.0	58.0	57.9	4.8	0.0	0.101515	21105	21817	1148-1163	0.363636	58.8
trnG-UCC_p5	trnG-UCC	5	ATTGCTCTACTGGCATCACG	TTTCCTCCTCCATTCTCGGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101991	21105	21817	1267-1282	0.363636	58.8
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	GTCCAGAAATCGTACCACCA	GGTTGTGGGTACGACTTCTG	20	20	50.0	55.0	57.2	58.2	0.0	0.0	0.962940	42882	43001	747-909	0.272727	53.2
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	GTCCAGAAATCGTACCACCA	GTTGTGGGTACGACTTCTGG	20	20	50.0	55.0	57.2	58.2	0.0	0.0	0.962940	42882	43001	746-908	0.272727	53.2
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	GTCCAGAAATCGTACCACCA	CCATAGGTTGCAGGTACGAC	20	20	50.0	55.0	57.2	58.1	0.0	0.0	0.819014	42882	43001	586-743	0.181818	47.5
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	TGTCCAGAAATCGTACCACC	CCATAGGTTGCAGGTACGAC	20	20	50.0	55.0	57.2	58.1	0.0	0.0	0.819014	42882	43001	587-744	0.181818	47.5
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GTCCAGAAACTGTACGGTCA	CCATAGGTTGCAGGTACGAC	20	20	50.0	55.0	57.2	58.1	8.0	0.0	0.870676	42882	43001	710-862	0.181818	46.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TGTAGCACCGTGAATAGCAC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.0	58.0	1.6	0.0	0.030171	48350	49253	1853-1927	0.818182	81.8
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TGTAGCACCGTGAATAGCAC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.0	58.0	1.6	4.2	0.042789	48350	49253	1817-1891	0.818182	81.7
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	ACCGATTAGACCGAAAGCAC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031559	48350	49253	1574-1648	0.636364	74.3
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	GATTAGACCGAAAGCACCGT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.031559	48350	49253	1571-1645	0.636364	74.3
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	GATTAGACCGAAAGCACCGT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.0	58.0	0.0	4.2	0.044177	48350	49253	1535-1609	0.636364	74.2
psaA-trnS-GGA_p1	psaA-trnS-GGA	1	AATCTTGACTTCCGGTTCCG	ATTCGAACCCTCGGTAAACG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.095191	54640	57054	2501-2635	1.000000	89.6
psaA-trnS-GGA_p2	psaA-trnS-GGA	2	TGACCCACTATTGGCCAAAC	ATTCGAACCCTCGGTAAACG	20	20	50.0	50.0	58.1	58.0	24.7	0.0	0.082557	54640	57054	2827-2961	1.000000	89.4
psaA-trnS-GGA_p3	psaA-trnS-GGA	3	GTTATTCCAGATGCTCGCCA	CTACACCTTCAACCACTCGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.126727	54640	57054	2872-2973	0.909091	85.4
psaA-trnS-GGA_p4	psaA-trnS-GGA	4	GTTATTCCAGATGCTCGCCA	ATTCGAACCCTCGGTAAACG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046870	54640	57054	2917-3000	0.454545	67.5
psaA-trnS-GGA_p5	psaA-trnS-GGA	5	TGGTGCAGCTTTGTGGTAAT	CTACACCTTCAACCACTCGG	20	20	45.0	55.0	58.0	57.9	18.1	0.0	0.086330	54640	57054	2969-2994	0.272727	53.4
atpB-rbcL_p1	atpB-rbcL	1	CGACGATAAGGAGCCAACAA	AATCCAACACCTGCTTTGGT	20	20	50.0	45.0	58.0	57.9	0.0	0.5	0.091193	64748	65287	1051-1096	1.000000	89.1
atpB-rbcL_p2	atpB-rbcL	2	CGACGATAAGGAGCCAACAA	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006913	64748	65287	1668-1713	1.000000	88.7
atpB-rbcL_p3	atpB-rbcL	3	CCCGCTCCTCCAAATAATCC	AGAAACGATCTCTCCAACGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.104771	64748	65287	1701-1746	1.000000	87.8
atpB-rbcL_p4	atpB-rbcL	4	CGACGATAAGGAGCCAACAA	CATGTACCTGCAGTAGCGTT	20	20	50.0	50.0	58.0	58.0	0.0	13.8	0.014728	64748	65287	1753-1757	0.363636	57.6
atpB-rbcL_p5	atpB-rbcL	5	CCCGCTCCTCCAAATAATCC	CATGTACCTGCAGTAGCGTT	20	20	55.0	50.0	58.1	58.0	0.0	13.8	0.112585	64748	65287	1786-1790	0.363636	56.8
petA-psbJ_p1	petA-psbJ	1	CAAGATCCTTCGCGAGTTCA	TTCCATTAATCACAGGCCGG	20	20	50.0	50.0	58.0	57.9	9.6	0.0	0.055043	72404	73518	1798-1817	1.000000	88.0
petA-psbJ_p2	petA-psbJ	2	CAAGATCCTTCGCGAGTTCA	GCCAATACGACCGGAAGAAT	20	20	50.0	50.0	58.0	58.1	9.6	9.5	0.054243	72404	73518	1358-1380	1.000000	87.8
petA-psbJ_p3	petA-psbJ	3	CGAGGTCGAGGGCAAATTTA	TTCCATTAATCACAGGCCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.069876	72404	73518	2074-2093	1.000000	87.8
petA-psbJ_p4	petA-psbJ	4	CAAGATCCTTCGCGAGTTCA	ACAGGCCGGTTCAATTCATT	20	20	50.0	45.0	58.0	58.1	9.6	1.3	0.080353	72404	73518	1787-1806	1.000000	87.8
petA-psbJ_p5	petA-psbJ	5	CGAGGTCGAGGGCAAATTTA	GCCAATACGACCGGAAGAAT	20	20	50.0	50.0	58.0	58.1	0.0	9.5	0.069077	72404	73518	1634-1656	1.000000	87.6
rps12-clpP_p1	rps12-clpP	1	AAAGCCCTCAGGACTTGTTG	CCGGAGCCGTATGATTTGAA	20	20	50.0	50.0	58.0	58.1	0.0	8.3	0.065013	77189	78179	1708-1992	0.909091	87.8
rps12-clpP_p2	rps12-clpP	2	AAAGCCCTCAGGACTTGTTG	AAGCCAGAGACTACGGACTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087009	77189	78179	1162-1445	0.909091	87.7
rps12-clpP_p3	rps12-clpP	3	AAAGCCCTCAGGACTTGTTG	GTATGGGGTTAGCTGCATCC	20	20	50.0	55.0	58.0	58.1	0.0	1.0	0.115558	77189	78179	1426-1709	0.909091	87.4
rps12-clpP_p4	rps12-clpP	4	AAAGCCCTCAGGACTTGTTG	ATGTCAGCAGAAGAAGCCAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.124019	77189	78179	1175-1458	0.909091	87.4
rps12-clpP_p5	rps12-clpP	5	AAAGCCCTCAGGACTTGTTG	TTCATCAACCCGCTAGTTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.017700	77189	78179	1580-1618	0.272727	62.4
psbH-petB_p1	psbH-petB	1	ATAGCGATCAAAGTTGCGGT	AAAGCTTCAGTAACCGTGGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.131966	82355	83274	1279-1306	0.909091	84.5
psbH-petB_p2	psbH-petB	2	CGATCAAAGTTGCGGTTTCC	AAAGCTTCAGTAACCGTGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144044	82355	83274	1275-1302	0.909091	84.5
psbH-petB_p3	psbH-petB	3	ATAGCGATCAAAGTTGCGGT	ACCAATCTGATCCCACGGTA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.150046	82355	83274	1514-1542	0.909091	84.2
psbH-petB_p4	psbH-petB	4	CGATCAAAGTTGCGGTTTCC	ACCAATCTGATCCCACGGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.162124	82355	83274	1510-1538	0.909091	84.1
psbH-petB_p5	psbH-petB	5	ATAGCGATCAAAGTTGCGGT	TCAGTAACCGTGGGACGATA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.151446	82355	83274	1273-1295	0.545455	69.5