Hotspot

ycf1

SSC · 131872-137466 · 5595 bp

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Pi 0.207

MarkerSeek score 77.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 1101
pi: 0.207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAGGAGAACCAAAAGGGAG	AAGAGGTTCCTCGATGGTCA	55.0	50.0	57.9	58.1	916 bp	0.5	58.7
ycf1_p2	CGAGGAGAACCAAAAGGGAG	CAAATGTGCATTCGCCACTT	55.0	45.0	57.9	57.9	622 bp	0.5	58.6
ycf1_p3	CGAGGAGAACCAAAAGGGAG	GAAGTCCCATTGGAATCCCC	55.0	55.0	57.9	57.9	1118 bp	0.5	58.6
ycf1_p4	CGAGGAGAACCAAAAGGGAG	ACTTCTACAGATGCGGAGGA	55.0	50.0	57.9	57.9	1274 bp	0.5	58.0
ycf1_p5	CGAGGAGAACCAAAAGGGAG	TTCTACAGATGCGGAGGAGT	55.0	50.0	57.9	57.9	1272 bp	0.5	58.0