Hotspot

clpP

LSC · 75024-77130 · 2107 bp

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Pi 0.347

MarkerSeek score 72.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2107
variable_sites: 397
pi: 0.347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTGGCTGTCTTGCGTTTC	TGTGAACAGAAAGCAACCGA	50.0	45.0	58.0	58.0	2088-2842 bp	1.0	91.5
clpP_p2	GATTGGCTGTCTTGCGTTTC	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2203-2957 bp	1.0	91.2
clpP_p3	GATTGGCTGTCTTGCGTTTC	ACATTGGATCAAGAACGGGG	50.0	50.0	58.0	57.9	2184-2938 bp	1.0	90.7
clpP_p4	GATTGGCTGTCTTGCGTTTC	CATTGGATCAAGAACGGGGT	50.0	50.0	58.0	57.9	2183-2937 bp	1.0	90.7
clpP_p5	GATTGGCTGTCTTGCGTTTC	CGCAATGGGGAATTGGTTTC	50.0	50.0	58.0	58.0	2555 bp	0.5	59.7