Hotspot

accD

LSC · 61451-62923 · 1473 bp

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Pi 0.236

MarkerSeek score 81.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1473
variable_sites: 287
pi: 0.236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAGACCCTAGCTCAAACCA	TGGTCACGATGAATTACTGCT	50.0	42.9	58.0	57.4	2004 bp	0.5	46.9
accD_p2	TTTTCGTTCCGTGTTCCCAT	TGGTCACGATGAATTACTGCT	45.0	42.9	58.0	57.4	1948 bp	0.5	46.9
accD_p3	GACCCTAGCTCAAACCAAGG	TGGTCACGATGAATTACTGCT	55.0	42.9	57.9	57.4	2001 bp	0.5	46.2
accD_p4	AATGATACCCCGGATTCCCA	TGGTCACGATGAATTACTGCT	50.0	42.9	58.2	57.4	1751 bp	0.5	45.7
accD_p5	ACCAAGGGAAGGTGTCTTCT	TGGTCACGATGAATTACTGCT	50.0	42.9	58.2	57.4	1988 bp	0.5	45.7