Hotspot

ndhB-tRNA-Leu(CAA)

IRa · 152190-153172 · 983 bp

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Pi 0.111

MarkerSeek score 84.00

Primer available yes

Label ndhB-tRNA-Leu(CAA)

Feature Metadata

label_name: ndhB-tRNA-Leu(CAA)
feature_type: igs
region: IRa
length_bp: 983
variable_sites: 86
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-tRNA-Leu(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	853-1043 bp	1.0	90.7
ndhB-tRNA-Leu(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	851-1041 bp	1.0	90.7
ndhB-tRNA-Leu(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	848-1038 bp	1.0	89.3
ndhB-tRNA-Leu(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	852-1042 bp	1.0	88.6
ndhB-tRNA-Leu(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	850-1040 bp	1.0	88.5