Hotspot

psaC-ndhE

SSC · 123543-124525 · 983 bp

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Pi 0.205

MarkerSeek score 84.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 179
pi: 0.205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTGA	55.0	28.0	58.4	55.3	1076-1103 bp	1.0	52.0
psaC-ndhE_p2	GCCCGTACACATTGAGTACA	CGTATCAATCAATCAAATTTGTTGA	50.0	28.0	57.6	55.3	1075-1102 bp	1.0	52.0
psaC-ndhE_p3	AGGCCCGTACACATTGAGTA	CGTATCAATCAATCAAATTTGTTGA	50.0	28.0	58.4	55.3	1077-1104 bp	1.0	52.0
psaC-ndhE_p4	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTG	55.0	29.2	58.4	53.9	1076-1103 bp	1.0	52.0
psaC-ndhE_p5	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTGAA	55.0	26.9	58.4	55.9	1076-1103 bp	1.0	52.0