Hotspot

psbE-petL

LSC · 69757-70935 · 1179 bp

Back to Epipremnum

Pi 0.16

MarkerSeek score 85.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 174
pi: 0.16
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTGACGAATAACCAACCCG	ACCAAAATAACTAGTTATAGTAGGCA	50.0	30.8	57.9	56.0	1323-1360 bp	1.0	52.0
psbE-petL_p2	ACGAATAACCAACCCGCAAT	ACCAAAATAACTAGTTATAGTAGGCA	45.0	30.8	58.2	56.0	1319-1356 bp	1.0	52.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	AACCAAAATAACTAGTTATAGTAGGCA	50.0	29.6	57.9	56.6	1324-1361 bp	1.0	52.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AACCAAAATAACTAGTTATAGTAGGCA	45.0	29.6	58.2	56.6	1320-1357 bp	1.0	52.0
psbE-petL_p5	ACTGACGAATAACCAACCCG	CCAAAATAACTAGTTATAGTAGGCA	50.0	32.0	57.9	54.4	1322-1359 bp	1.0	52.0