Hotspot

accD-psaI

LSC · 62924-63992 · 1069 bp

Back to Epipremnum

Pi 0.183

MarkerSeek score 82.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 177
pi: 0.183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CATTCGACTGGAGTTGGGAG	50.0	55.0	58.0	58.0	1663 bp	0.5	59.8
accD-psaI_p2	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1259 bp	0.5	59.7
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	CCGCTTTTGACCAGAGCTAT	50.0	50.0	58.0	58.0	1515 bp	0.5	59.7
accD-psaI_p4	TCACAAGCGGCTGAGTATTC	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1740 bp	0.5	59.6
accD-psaI_p5	GCGAGAAAATCACCCGTTTG	CATTCGACTGGAGTTGGGAG	50.0	55.0	58.0	58.0	1983 bp	0.5	59.6