Hotspot

atpB-rbcL

LSC · 58445-59241 · 797 bp

Back to Epipremnum

Pi 0.15

MarkerSeek score 83.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 109
pi: 0.15
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATTCGTCCGAGAGTTGCTC	ATCCTCCAGACGTAGAGCTC	50.0	55.0	58.0	58.0	1479-1538 bp	1.0	91.0
atpB-rbcL_p2	AATTCGTCCGAGAGTTGCTC	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	1573-1632 bp	1.0	90.8
atpB-rbcL_p3	AATTCGTCCGAGAGTTGCTC	CTACCGTAGTTTTTCGCGGA	50.0	50.0	58.0	58.0	1628-1687 bp	1.0	90.4
atpB-rbcL_p4	AATTCGTCCGAGAGTTGCTC	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1718-1777 bp	1.0	90.0
atpB-rbcL_p5	TTTTCCTCCACGGCGATAAG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	1889-1948 bp	1.0	89.0