Hotspot

ndhC-tRNA-Val(UAC)

LSC · 54328-55468 · 1141 bp

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Pi 0.212

MarkerSeek score 78.00

Primer available yes

Label ndhC-tRNA-Val(UAC)

Feature Metadata

label_name: ndhC-tRNA-Val(UAC)
feature_type: igs
region: LSC
length_bp: 1141
variable_sites: 173
pi: 0.212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Val(UAC)_p1	AGAGAAGCGGCCATAAACTG	GAGCAAATGGAGCCATCTCA	50.0	50.0	58.0	58.0	1582-1881 bp	1.0	91.4
ndhC-tRNA-Val(UAC)_p2	AGAGAAGCGGCCATAAACTG	TCAGAGCAAATGGAGCCATC	50.0	50.0	58.0	58.0	1585-1884 bp	1.0	91.4
ndhC-tRNA-Val(UAC)_p3	AGAGAAGCGGCCATAAACTG	CAGAGCAAATGGAGCCATCT	50.0	50.0	58.0	57.9	1584-1883 bp	1.0	91.3
ndhC-tRNA-Val(UAC)_p4	ACTAGAGAGCTTCTCCGGTC	GAGCAAATGGAGCCATCTCA	55.0	50.0	58.0	58.0	1177-1473 bp	1.0	91.2
ndhC-tRNA-Val(UAC)_p5	ACTAGAGAGCTTCTCCGGTC	TCAGAGCAAATGGAGCCATC	55.0	50.0	58.0	58.0	1180-1476 bp	1.0	91.2