Hotspot

tRNA-Cys(GCA)-petN

LSC · 29440-30406 · 967 bp

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Pi 0.094

MarkerSeek score 86.30

Primer available yes

Label tRNA-Cys(GCA)-petN

Feature Metadata

label_name: tRNA-Cys(GCA)-petN
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 82
pi: 0.094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Cys(GCA)-petN_p1	AGTTCAAATCTGGGTGTCGC	CTCCTCCCCCATACTACGAG	50.0	60.0	58.5	58.1	1021-1069 bp	1.0	85.8
tRNA-Cys(GCA)-petN_p2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	968-1016 bp	1.0	85.6
tRNA-Cys(GCA)-petN_p3	GGGGGACTGCAAATCCTTTA	CTCCTCCCCCATACTACGAG	50.0	60.0	57.5	58.1	1046-1094 bp	1.0	85.2
tRNA-Cys(GCA)-petN_p4	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	993-1041 bp	1.0	85.0
tRNA-Cys(GCA)-petN_p5	AGTTCAAATCTGGGTGTCGC	TCTAAAGTCCACTCCTCCCC	50.0	55.0	58.5	57.8	1032-1080 bp	1.0	84.4