Hotspot

tRNA-Ser(GCU)-tRNA-Ser (CGA)

LSC · 9078-9956 · 879 bp

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Pi 0.219

MarkerSeek score 84.60

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Ser (CGA)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Ser (CGA)
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 185
pi: 0.219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p1	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	55.0	40.9	58.5	57.7	937-1629 bp	1.0	67.4
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p2	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	55.0	45.5	58.5	57.6	936-1628 bp	1.0	66.9
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p3	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.2	57.7	938-1630 bp	1.0	64.8
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p4	TAGTCCACTCAGCCATCTCT	GGACGAATCACACTTTTACCAC	50.0	45.5	57.2	57.6	937-1629 bp	1.0	64.4
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p5	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCA	55.0	42.9	58.5	56.2	936-1628 bp	1.0	63.7