primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys(UUU)-rps16_p1	tRNA-Lys(UUU)-rps16	1	CCTTTCAGGATCAGTCGTGG	AAAGGAGCTCAACCCACAAG	20	20	55.0	50.0	58.0	58.0	0.0	17.2	0.028853	4383	5049	860-950	1.000000	89.7
tRNA-Lys(UUU)-rps16_p2	tRNA-Lys(UUU)-rps16	2	CCTTTCAGGATCAGTCGTGG	AGCTCAACCCACAAGAACTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.042864	4383	5049	855-945	1.000000	89.6
tRNA-Lys(UUU)-rps16_p3	tRNA-Lys(UUU)-rps16	3	TAAGAACAGAAGCTCGTCGC	AAAGGAGCTCAACCCACAAG	20	20	50.0	50.0	58.0	58.0	0.0	17.2	0.034576	4383	5049	1069-1169	1.000000	89.2
tRNA-Lys(UUU)-rps16_p4	tRNA-Lys(UUU)-rps16	4	TAAGAACAGAAGCTCGTCGC	AGCTCAACCCACAAGAACTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048586	4383	5049	1064-1164	1.000000	89.2
tRNA-Lys(UUU)-rps16_p5	tRNA-Lys(UUU)-rps16	5	CCTTTCAGGATCAGTCGTGG	AGAGATCTTCGGAGAGTGGG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.081634	4383	5049	944-1034	1.000000	89.1
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p1	tRNA-Ser(GCU)-tRNA-Ser (CGA)	1	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	20	22	55.0	40.9	58.5	57.7	0.0	0.0	2.861821	9078	9956	937-1629	1.000000	67.4
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p2	tRNA-Ser(GCU)-tRNA-Ser (CGA)	2	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	20	22	55.0	45.5	58.5	57.6	0.0	0.0	2.915998	9078	9956	936-1628	1.000000	66.9
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p3	tRNA-Ser(GCU)-tRNA-Ser (CGA)	3	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	20	22	50.0	40.9	57.2	57.7	0.0	0.0	3.182495	9078	9956	938-1630	1.000000	64.8
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p4	tRNA-Ser(GCU)-tRNA-Ser (CGA)	4	TAGTCCACTCAGCCATCTCT	GGACGAATCACACTTTTACCAC	20	22	50.0	45.5	57.2	57.6	0.0	0.0	3.236672	9078	9956	937-1629	1.000000	64.4
tRNA-Ser(GCU)-tRNA-Ser (CGA)_p5	tRNA-Ser(GCU)-tRNA-Ser (CGA)	5	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCA	20	21	55.0	42.9	58.5	56.2	0.0	0.0	3.319052	9078	9956	936-1628	1.000000	63.7
tRNA-Cys(GCA)-petN_p1	tRNA-Cys(GCA)-petN	1	AGTTCAAATCTGGGTGTCGC	CTCCTCCCCCATACTACGAG	20	20	50.0	60.0	58.5	58.1	0.0	0.0	0.563826	29440	30406	1021-1069	1.000000	85.8
tRNA-Cys(GCA)-petN_p2	tRNA-Cys(GCA)-petN	2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	29440	30406	968-1016	1.000000	85.6
tRNA-Cys(GCA)-petN_p3	tRNA-Cys(GCA)-petN	3	GGGGGACTGCAAATCCTTTA	CTCCTCCCCCATACTACGAG	20	20	50.0	60.0	57.5	58.1	14.7	0.0	0.612688	29440	30406	1046-1094	1.000000	85.2
tRNA-Cys(GCA)-petN_p4	tRNA-Cys(GCA)-petN	4	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.5	58.2	14.7	0.0	0.676453	29440	30406	993-1041	1.000000	85.0
tRNA-Cys(GCA)-petN_p5	tRNA-Cys(GCA)-petN	5	AGTTCAAATCTGGGTGTCGC	TCTAAAGTCCACTCCTCCCC	20	20	50.0	55.0	58.5	57.8	0.0	0.0	0.725551	29440	30406	1032-1080	1.000000	84.4
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1	tRNA-Glu(UUC)-tRNA-Thr(GGU)	1	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	33497	34527	785-1095	1.000000	90.3
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2	tRNA-Glu(UUC)-tRNA-Thr(GGU)	2	AGATGTCCTGAACCGCTAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.231619	33497	34527	782-1092	1.000000	90.1
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3	tRNA-Glu(UUC)-tRNA-Thr(GGU)	3	GATGTCCTGAACCGCTAGAC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.275420	33497	34527	784-1094	1.000000	89.8
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4	tRNA-Glu(UUC)-tRNA-Thr(GGU)	4	GATGTCCTGAACCGCTAGAC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.297575	33497	34527	781-1091	1.000000	89.6
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5	tRNA-Glu(UUC)-tRNA-Thr(GGU)	5	GAGATGTCCTGAACCGCTAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.5	58.1	0.0	0.0	0.564576	33497	34527	786-1096	1.000000	87.5
ycf3-tRNA-Ser(GGA)_p1	ycf3-tRNA-Ser(GGA)	1	ACACGACAGAAGGCATTGTT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.127044	47358	48236	1236-1253	1.000000	89.8
ycf3-tRNA-Ser(GGA)_p2	ycf3-tRNA-Ser(GGA)	2	GTCATCCTTCCTGCAAGAGG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.0	58.1	3.8	0.0	0.129893	47358	48236	1643-1656	1.000000	88.1
ycf3-tRNA-Ser(GGA)_p3	ycf3-tRNA-Ser(GGA)	3	TTTCTTTGTCCCTAACGCCC	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.194117	47358	48236	1568-1589	1.000000	87.4
ycf3-tRNA-Ser(GGA)_p4	ycf3-tRNA-Ser(GGA)	4	ACACGACAGAAGGCATTGTT	GGACAATTTTGCCCGCTTTT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.155045	47358	48236	1558-1558	0.500000	58.8
ycf3-tRNA-Ser(GGA)_p5	ycf3-tRNA-Ser(GGA)	5	GTCATCCTTCCTGCAAGAGG	GGACAATTTTGCCCGCTTTT	20	20	55.0	45.0	58.0	58.1	3.8	0.0	0.157893	47358	48236	1965-1965	0.500000	58.7
ndhC-tRNA-Val(UAC)_p1	ndhC-tRNA-Val(UAC)	1	AGAGAAGCGGCCATAAACTG	GAGCAAATGGAGCCATCTCA	20	20	50.0	50.0	58.0	58.0	2.2	1.9	0.078282	54328	55468	1582-1881	1.000000	91.4
ndhC-tRNA-Val(UAC)_p2	ndhC-tRNA-Val(UAC)	2	AGAGAAGCGGCCATAAACTG	TCAGAGCAAATGGAGCCATC	20	20	50.0	50.0	58.0	58.0	2.2	1.9	0.078282	54328	55468	1585-1884	1.000000	91.4
ndhC-tRNA-Val(UAC)_p3	ndhC-tRNA-Val(UAC)	3	AGAGAAGCGGCCATAAACTG	CAGAGCAAATGGAGCCATCT	20	20	50.0	50.0	58.0	57.9	2.2	1.9	0.084070	54328	55468	1584-1883	1.000000	91.3
ndhC-tRNA-Val(UAC)_p4	ndhC-tRNA-Val(UAC)	4	ACTAGAGAGCTTCTCCGGTC	GAGCAAATGGAGCCATCTCA	20	20	55.0	50.0	58.0	58.0	0.0	1.9	0.095446	54328	55468	1177-1473	1.000000	91.2
ndhC-tRNA-Val(UAC)_p5	ndhC-tRNA-Val(UAC)	5	ACTAGAGAGCTTCTCCGGTC	TCAGAGCAAATGGAGCCATC	20	20	55.0	50.0	58.0	58.0	0.0	1.9	0.095446	54328	55468	1180-1476	1.000000	91.2
atpB-rbcL_p1	atpB-rbcL	1	AATTCGTCCGAGAGTTGCTC	ATCCTCCAGACGTAGAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	5.3	0.026671	58445	59241	1479-1538	1.000000	91.0
atpB-rbcL_p2	atpB-rbcL	2	AATTCGTCCGAGAGTTGCTC	GGGGACGACCATACTTGTTC	20	20	50.0	55.0	58.0	58.0	0.0	6.2	0.022279	58445	59241	1573-1632	1.000000	90.8
atpB-rbcL_p3	atpB-rbcL	3	AATTCGTCCGAGAGTTGCTC	CTACCGTAGTTTTTCGCGGA	20	20	50.0	50.0	58.0	58.0	0.0	6.1	0.021077	58445	59241	1628-1687	1.000000	90.4
atpB-rbcL_p4	atpB-rbcL	4	AATTCGTCCGAGAGTTGCTC	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.006913	58445	59241	1718-1777	1.000000	90.0
atpB-rbcL_p5	atpB-rbcL	5	TTTTCCTCCACGGCGATAAG	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.023125	58445	59241	1889-1948	1.000000	89.0
accD_p1	accD	1	TCAGACCCTAGCTCAAACCA	TGGTCACGATGAATTACTGCT	20	21	50.0	42.9	58.0	57.4	0.0	0.0	1.634216	61451	62923	2004-2004	0.500000	46.9
accD_p2	accD	2	TTTTCGTTCCGTGTTCCCAT	TGGTCACGATGAATTACTGCT	20	21	45.0	42.9	58.0	57.4	0.0	0.0	1.639948	61451	62923	1948-1948	0.500000	46.9
accD_p3	accD	3	GACCCTAGCTCAAACCAAGG	TGGTCACGATGAATTACTGCT	20	21	55.0	42.9	57.9	57.4	0.0	0.0	1.725383	61451	62923	2001-2001	0.500000	46.2
accD_p4	accD	4	AATGATACCCCGGATTCCCA	TGGTCACGATGAATTACTGCT	20	21	50.0	42.9	58.2	57.4	0.7	0.0	1.782248	61451	62923	1751-1751	0.500000	45.7
accD_p5	accD	5	ACCAAGGGAAGGTGTCTTCT	TGGTCACGATGAATTACTGCT	20	21	50.0	42.9	58.2	57.4	6.2	0.0	1.788952	61451	62923	1988-1988	0.500000	45.7
accD-psaI_p1	accD-psaI	1	TCACAAGCGGCTGAGTATTC	CATTCGACTGGAGTTGGGAG	20	20	50.0	55.0	58.0	58.0	1.8	0.0	0.025881	62924	63992	1663-1663	0.500000	59.8
accD-psaI_p2	accD-psaI	2	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	1.8	26.9	0.037148	62924	63992	1259-1259	0.500000	59.7
accD-psaI_p3	accD-psaI	3	TCACAAGCGGCTGAGTATTC	CCGCTTTTGACCAGAGCTAT	20	20	50.0	50.0	58.0	58.0	1.8	0.0	0.039983	62924	63992	1515-1515	0.500000	59.7
accD-psaI_p4	accD-psaI	4	TCACAAGCGGCTGAGTATTC	TTGTTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	1.8	0.0	0.051396	62924	63992	1740-1740	0.500000	59.6
accD-psaI_p5	accD-psaI	5	GCGAGAAAATCACCCGTTTG	CATTCGACTGGAGTTGGGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051989	62924	63992	1983-1983	0.500000	59.6
psbE-petL_p1	psbE-petL	1	ACTGACGAATAACCAACCCG	ACCAAAATAACTAGTTATAGTAGGCA	20	26	50.0	30.8	57.9	56.0	0.0	24.9	8.114200	69757	70935	1323-1360	1.000000	52.0
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	ACCAAAATAACTAGTTATAGTAGGCA	20	26	45.0	30.8	58.2	56.0	0.0	24.9	8.196271	69757	70935	1319-1356	1.000000	52.0
psbE-petL_p3	psbE-petL	3	ACTGACGAATAACCAACCCG	AACCAAAATAACTAGTTATAGTAGGCA	20	27	50.0	29.6	57.9	56.6	0.0	24.9	8.499475	69757	70935	1324-1361	1.000000	52.0
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AACCAAAATAACTAGTTATAGTAGGCA	20	27	45.0	29.6	58.2	56.6	0.0	24.9	8.581546	69757	70935	1320-1357	1.000000	52.0
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	CCAAAATAACTAGTTATAGTAGGCA	20	25	50.0	32.0	57.9	54.4	0.0	24.9	8.657328	69757	70935	1322-1359	1.000000	52.0
clpP_p1	clpP	1	GATTGGCTGTCTTGCGTTTC	TGTGAACAGAAAGCAACCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.062155	75024	77130	2088-2842	1.000000	91.5
clpP_p2	clpP	2	GATTGGCTGTCTTGCGTTTC	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.097875	75024	77130	2203-2957	1.000000	91.2
clpP_p3	clpP	3	GATTGGCTGTCTTGCGTTTC	ACATTGGATCAAGAACGGGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.159667	75024	77130	2184-2938	1.000000	90.7
clpP_p4	clpP	4	GATTGGCTGTCTTGCGTTTC	CATTGGATCAAGAACGGGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.159667	75024	77130	2183-2937	1.000000	90.7
clpP_p5	clpP	5	GATTGGCTGTCTTGCGTTTC	CGCAATGGGGAATTGGTTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.7	0.039648	75024	77130	2555-2555	0.500000	59.7
tRNA-Leu(CAA)-ndhB_p1	tRNA-Leu(CAA)-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	99878	100860	853-1043	1.000000	90.7
tRNA-Leu(CAA)-ndhB_p2	tRNA-Leu(CAA)-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	99878	100860	851-1041	1.000000	90.7
tRNA-Leu(CAA)-ndhB_p3	tRNA-Leu(CAA)-ndhB	3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	99878	100860	848-1038	1.000000	89.3
tRNA-Leu(CAA)-ndhB_p4	tRNA-Leu(CAA)-ndhB	4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	57.7	57.9	5.8	29.4	0.330507	99878	100860	852-1042	1.000000	88.6
tRNA-Leu(CAA)-ndhB_p5	tRNA-Leu(CAA)-ndhB	5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.3	57.9	5.8	29.4	0.351881	99878	100860	850-1040	1.000000	88.5
tRNA-Asn(GUU)-ndhF_p1	tRNA-Asn(GUU)-ndhF	1	CGGGAGAATTGATGACTGCA	TTTCTTTGCGGGTGAAGGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.088470	115164	115830	1142-1535	1.000000	90.5
tRNA-Asn(GUU)-ndhF_p2	tRNA-Asn(GUU)-ndhF	2	ACCTGGGGCTTTTTACAAGG	TTTCTTTGCGGGTGAAGGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.069732	115164	115830	1069-1069	0.500000	59.4
tRNA-Asn(GUU)-ndhF_p3	tRNA-Asn(GUU)-ndhF	3	ACCTGGGGCTTTTTACAAGG	GGGGCGAATCTCGTCTTATC	20	20	50.0	55.0	58.0	57.9	0.0	0.3	0.077095	115164	115830	1030-1030	0.500000	59.4
tRNA-Asn(GUU)-ndhF_p4	tRNA-Asn(GUU)-ndhF	4	ACAGGGAACGGGTTAGGTTA	TTTCTTTGCGGGTGAAGGAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.088612	115164	115830	955-955	0.500000	59.3
tRNA-Asn(GUU)-ndhF_p5	tRNA-Asn(GUU)-ndhF	5	CGGGAGAATTGATGACTGCA	GGGGCGAATCTCGTCTTATC	20	20	50.0	55.0	58.0	57.9	0.0	0.3	0.095834	115164	115830	1103-1103	0.500000	59.2
psaC-ndhE_p1	psaC-ndhE	1	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTGA	20	25	55.0	28.0	58.4	55.3	12.7	24.9	8.101191	123543	124525	1076-1103	1.000000	52.0
psaC-ndhE_p2	psaC-ndhE	2	GCCCGTACACATTGAGTACA	CGTATCAATCAATCAAATTTGTTGA	20	25	50.0	28.0	57.6	55.3	19.8	24.9	8.123235	123543	124525	1075-1102	1.000000	52.0
psaC-ndhE_p3	psaC-ndhE	3	AGGCCCGTACACATTGAGTA	CGTATCAATCAATCAAATTTGTTGA	20	25	50.0	28.0	58.4	55.3	0.0	24.9	8.185771	123543	124525	1077-1104	1.000000	52.0
psaC-ndhE_p4	psaC-ndhE	4	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTG	20	24	55.0	29.2	58.4	53.9	12.7	17.5	8.434962	123543	124525	1076-1103	1.000000	52.0
psaC-ndhE_p5	psaC-ndhE	5	GGCCCGTACACATTGAGTAC	CGTATCAATCAATCAAATTTGTTGAA	20	26	55.0	26.9	58.4	55.9	12.7	25.8	8.456443	123543	124525	1076-1103	1.000000	52.0
ycf1_p1	ycf1	1	CGAGGAGAACCAAAAGGGAG	AAGAGGTTCCTCGATGGTCA	20	20	55.0	50.0	57.9	58.1	0.0	12.4	0.162020	135180	135299	916-916	0.500000	58.7
ycf1_p2	ycf1	2	CGAGGAGAACCAAAAGGGAG	CAAATGTGCATTCGCCACTT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.169442	135180	135299	622-622	0.500000	58.6
ycf1_p3	ycf1	3	CGAGGAGAACCAAAAGGGAG	GAAGTCCCATTGGAATCCCC	20	20	55.0	55.0	57.9	57.9	0.0	3.6	0.173857	135180	135299	1118-1118	0.500000	58.6
ycf1_p4	ycf1	4	CGAGGAGAACCAAAAGGGAG	ACTTCTACAGATGCGGAGGA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.246002	135180	135299	1274-1274	0.500000	58.0
ycf1_p5	ycf1	5	CGAGGAGAACCAAAAGGGAG	TTCTACAGATGCGGAGGAGT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.246002	135180	135299	1272-1272	0.500000	58.0
ndhB-tRNA-Leu(CAA)_p1	ndhB-tRNA-Leu(CAA)	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	152190	153172	853-1043	1.000000	90.7
ndhB-tRNA-Leu(CAA)_p2	ndhB-tRNA-Leu(CAA)	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	152190	153172	851-1041	1.000000	90.7
ndhB-tRNA-Leu(CAA)_p3	ndhB-tRNA-Leu(CAA)	3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	152190	153172	848-1038	1.000000	89.3
ndhB-tRNA-Leu(CAA)_p4	ndhB-tRNA-Leu(CAA)	4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	29.4	5.8	0.330507	152190	153172	852-1042	1.000000	88.6
ndhB-tRNA-Leu(CAA)_p5	ndhB-tRNA-Leu(CAA)	5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	20	20	50.0	50.0	57.9	58.3	29.4	5.8	0.351881	152190	153172	850-1040	1.000000	88.5