Hotspot

ndhA

SSC · 120444-122643 · 2200 bp

Back to Epipactis

Pi 0.0064

MarkerSeek score 41.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2200
variable_sites: 38
pi: 0.0064
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTGCAAATTCTTCCCCAGCT	TCGACACAGTTACCGCAAAA	45.0	45.0	58.0	58.1	2736-2758 bp	1.0	81.4
ndhA_p2	TTGCAAATTCTTCCCCAGCT	TCCAATGTACCTTGCAGCTC	45.0	50.0	58.0	57.9	2470-2492 bp	1.0	81.1
ndhA_p3	TAGAGGCCCCAAAAGGAGAA	TCGACACAGTTACCGCAAAA	50.0	45.0	57.9	58.1	2831-2853 bp	1.0	81.0
ndhA_p4	TAGTAGCGTTTTCCCCTGGA	TCGACACAGTTACCGCAAAA	50.0	45.0	58.1	58.1	2788-2810 bp	1.0	81.0
ndhA_p5	GCATTATTGGTGGGGAGGAG	TCCAATGTACCTTGCAGCTC	55.0	50.0	58.0	57.9	2934-2956 bp	1.0	80.9