Hotspot

trnL-UAG-rpl32

SSC · 128587-129291 · 705 bp

Back to Epipactis

Pi 0.0106

MarkerSeek score 56.00

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 705
variable_sites: 20
pi: 0.0106
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	57.9	58.0	789-804 bp	1.0	82.4
trnL-UAG-rpl32_p2	AGCAGCGTGTCTACCAATTT	TCCACCGGACAGTCAAAAAG	45.0	50.0	57.8	58.0	782-797 bp	1.0	81.9
trnL-UAG-rpl32_p3	CCTAAGAGCAGCGTGTCTAC	TCCACCGGACAGTCAAAAAG	55.0	50.0	57.8	58.0	788-803 bp	1.0	81.8
trnL-UAG-rpl32_p4	CTAAGAGCAGCGTGTCTACC	TCCACCGGACAGTCAAAAAG	55.0	50.0	57.8	58.0	787-802 bp	1.0	81.8
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	AATCTGTTTCCACCGGACAG	50.0	50.0	57.9	57.8	797-812 bp	1.0	81.2