Hotspot

clpP-psbB

LSC · 74342-75300 · 959 bp

Back to Epipactis

Pi 0.0128

MarkerSeek score 68.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 30
pi: 0.0128
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	965-1142 bp	1.0	85.3
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	960-1137 bp	1.0	85.1
clpP-psbB_p3	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	931-1108 bp	1.0	84.7
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	926-1103 bp	1.0	84.6
clpP-psbB_p5	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	883-1060 bp	1.0	84.5