Hotspot

psbE-petL

LSC · 66412-67759 · 1348 bp

Back to Epipactis

Pi 0.00898

MarkerSeek score 59.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1348
variable_sites: 32
pi: 0.00898
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1425-1516 bp	1.0	77.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1421-1512 bp	1.0	77.3
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1423-1514 bp	1.0	77.3
psbE-petL_p4	ACCAACCCGCAATGAATAGG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	58.2	58.0	1423-1514 bp	1.0	75.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	58.2	58.0	1425-1516 bp	1.0	75.8