Hotspot

petA-psbJ

LSC · 64814-65648 · 835 bp

Back to Epipactis

Pi 0.0123

MarkerSeek score 67.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 28
pi: 0.0123
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	947-1015 bp	1.0	80.5
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	948-1016 bp	1.0	80.5
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	972-1040 bp	1.0	79.0
petA-psbJ_p4	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	973-1041 bp	1.0	78.9
petA-psbJ_p5	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	970-1038 bp	1.0	78.2