Hotspot

atpB-rbcL

LSC · 55740-56723 · 984 bp

Back to Epipactis

Pi 0.0115

MarkerSeek score 62.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 34
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1034-1096 bp	1.0	84.3
atpB-rbcL_p2	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	1035-1097 bp	1.0	84.3
atpB-rbcL_p3	TGAGTGATACGCCCTAGGTT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	1035-1097 bp	1.0	81.9
atpB-rbcL_p4	TTGAGTGATACGCCCTAGGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	1036-1098 bp	1.0	81.9
atpB-rbcL_p5	TGAGTGATACGCCCTAGGTT	TCCAACGCTTGCTTTAGTCT	50.0	45.0	57.8	57.5	1062-1124 bp	1.0	80.9