Hotspot

trnE-UUC-trnT-GGU

LSC · 31264-32810 · 1547 bp

Back to Epipactis

Pi 0.0103

MarkerSeek score 53.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1547
variable_sites: 46
pi: 0.0103
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTAGTCTACTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	1577-1705 bp	1.0	86.0
trnE-UUC-trnT-GGU_p2	CGTAGTCTACTACCCCCAGG	ACTTGTGTGGTCCAATGGTC	60.0	50.0	58.0	58.0	2077-2203 bp	1.0	85.9
trnE-UUC-trnT-GGU_p3	CGTAGTCTACTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	1556-1684 bp	1.0	85.6
trnE-UUC-trnT-GGU_p4	AATTTTGTTCCCCGTCCTCC	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	2132-2258 bp	1.0	85.4
trnE-UUC-trnT-GGU_p5	GTGCTCTGACCGATTGAACT	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	2023-2150 bp	1.0	85.4